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24107

Supelco

Nukol Capillary GC Column

size × I.D. 30 m × 0.25 mm, df 0.25 μm

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About This Item

UNSPSC Code:
41115710
Pricing and availability is not currently available.

material

fused silica

Quality Level

100

Agency

meets requirements for USP G25 and G35

parameter

60-200 °C temperature (isothermal)
60-220 °C temperature (programmed)

Beta value

250

df

0.25 μm

technique(s)

gas chromatography (GC): suitable

size × I.D.

30 m × 0.25 mm

matrix active group

Bonded; acid-modified poly(ethylene glycol) phase

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
food and beverages
forensics and toxicology
personal care

column type

capillary polar

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General description

Application: The incorporation of acid functional groups into the phase lends an acidic character to this column, useful for analyses of volatile acidic compounds. Difficult to analyze carboxylic acids (free fatty acids) can be analyzed with excellent peak shape and minimal adsorption.
USP Code: This column meets USP G25 and G35 requirements.
Phase:
  • Bonded
  • Acid-modified poly(ethylene glycol)
Temp. Limits:
  • 60 °C to 200 °C (isothermal) or 220 °C (programmed)

Application

Nukol capillary GC column may be used for quantitative determination of free volatile fatty acids (FVFA) from dairy products.[1] It may also be used for the determination of the free concentration of valproic acid in human plasma by equilibrium dialysis followed by solid-phase microextraction (SPME) and capillary gas chromatography.[2]

Other Notes

We offer a variety of chromatography accessories including analytical syringes

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Certificates of Analysis (COA)

Lot/Batch Number
292776-08
292776-07
292776-06
292776-05
292776-04

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Solid-phase microextraction for the determination of the free concentration of valproic acid in human plasma by capillary gas chromatography.
Mette K
Journal of Chromatography. B, Biomedical Sciences and Applications, 673 (2), 299-305 (1995)
Quantitative determination of free volatile fatty acids from dairy products on a Nukol capillary column.
Luciano C.
Journal of Chromatography A, 519 (2), 369-378 (1990)

Protocols

GC Analyses of Free Fatty Acids

This article will discuss the analysis of free fatty acid.

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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