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ZRB1405

Sigma-Aldrich

Anti-SLFN11 Antibody, clone 3E13 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3E13, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 102.84 kDa
observed mol wt ~100 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

epitope sequence

N-terminal half

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 3E13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Schlafen family member 11 (SLFN11). It targets an epitope within the N-terminal half.

Immunogen

His-tagged recombinant fragment corresponding to 73 amino acids from the N-terminal half of human Schlafen family member 11 (SLFN11).

Application

Quality Control Testing

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLFN11 in HL-60 cell lysate.

Tested applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SLFN11 in HL-60 cells.

Affinity Binding Assay: A representative lot of this antibody bound recombinant SLFN11 fragment with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SLFN11, clone 3E13 ZooMAb®, Cat. No. ZRB1405, is a recombinant Rabbit monoclonal antibody that detects Schlafen family member 11 (SLFN11) and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.

Target description

Schlafen family member 11 (UniProt: Q7Z7L1; also known as SLFN11) is encoded by the SLFN11 gene (Gene ID: 91607) in human. SLFN11 is an inhibitor of DNA replication that promotes cell death in response to DNA damage. It is reported to block stressed replication forks by opening chromatin across replication initiation sites at stressed replication forks that leads to unwinding of DNA ahead of the minichromosome maintenance protein complex (MCM) helicase and block fork progression, ultimately leading to cell death. It is shown to act independently of ATR. SLFN11 is also reported to be a dominant determinant of sensitivity to DNA-damaging anticancer drugs. Its levels are down-regulated in a number of chemoresistant tumors. It is shown to be down-regulated in small cell lung cancer (SCLC) cells that are resistant to PARP inhibitor drugs. It exhibits a wider expression range in ovarian and colon adenocarcinoma than in their corresponding healthy tissues. Its levels are up-regulated by type I interferons and it acts as an interferon-induced antiviral protein that inhibits retrovirus protein synthesis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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