Skip to Content
Merck
All Photos(1)

Documents

T3934

Sigma-Aldrich

TRI Reagent®

LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.

Synonym(s):

DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, cell CSF amniotic fluid RNA extraction reagent, cell CSF amniotic fluid RNA isolation reagent, cell CSF amniotic fluid RNA purification reagent, single step RNA extraction reagent, single step RNA isolation reagent, single step RNA purification reagent, total RNA extraction solution, total RNA isolation solution, total RNA purification solution, TRI Reagent® RNA Isolation Reagent

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352200
NACRES:
NA.52

Quality Level

usage

0.75 mL sufficient for 0.25 mL fluid samples

Looking for similar products? Visit Product Comparison Guide

General description

TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein from liquid samples of human, animal, plant, yeast, bacterial, and viral origin.

A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes, and many samples can be simultaneously extracted.

TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution. When a biological sample is homogenized or lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI Reagent LS processes 0.25 ml of a liquid sample such as amniotic fluid.

This is one of the most effective methods for isolating total RNA from fresh samples in only one hour. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.

Application

TRI Reagent® has been used in RNA extraction.
The reagent has been used for isolating total RNA. The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Features and Benefits

  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Target Organs

Nervous system,Kidney,Liver,Skin

Supplementary Hazards

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

174.2 °F - closed cup

Flash Point(C)

79 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

TGF Presence During IgE-dependent Sensitization Primes Mast Cells for Higher VEGF Production After Fc RI Activation
Garrido
Open Allergy Journal (2009)
Nicola Jeffery et al.
Cell & bioscience, 11(1), 144-144 (2021-07-25)
Beta cell identity changes occur in the islets of donors with diabetes, but the molecular basis of this remains unclear. Protecting residual functional beta cells from cell identity changes may be beneficial for patients with diabetes. A somatostatin-positive cell population
TGF beta Presence During IgE-dependent Sensitization Primes Mast Cells for Higher VEGF Production After Fc varepsilon RI Activation
Benitez-GJP, et al.
Open Allergy Journal, 2, 16-26 (2009)
Raul Y Sanchez David et al.
eLife, 5, e11275-e11275 (2016-03-25)
The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate and modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown. To decipher the viral RNA signature on RLRs during
Cyclic dinucleotides modulate induced type I IFN responses in innate immune cells by degradation of STING
Rueckert C, et al.
Faseb Journal (2017)

Articles

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service