SRP3240

GRO/KC (CXCL1) from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): CINC, CXCL1, GRO?, NAP-3
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: rat
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 10.0-100.0 ng/mL
• mol wt: 7.8 kDa
• packaging: pkg of 25 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: P14095
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: rat ... CXCL1(81503)

Description

General description

All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant rat KC is a 7.8 kDa protein consisting of 72 amino acids including the ‘ELR′ motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

Biochem/physiol Actions

All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. Recombinant rat KC is a 7.8 kDa protein consisting of 72 amino acids including the ‘ELR′ motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

Sequence

APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable