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SAB5900206

Sigma-Aldrich

Alpaca Anti-EGFR, recombinant single domain Antibody

new

recombinant Alpaca (VHH) Nano antibody

Synonym(s):

ERBB, ERBB1, HER1, NISBD2, PIG61

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

alpaca

Quality Level

recombinant

expressed in E. coli

conjugate

unconjugated

antibody form

affinity purified VHH antibody

clone

EB61, monoclonal

Assay

>95% (SDS-PAGE)

form

lyophilized solid

mol wt

14.4 kDa
~15-18 kDa (SDS PAGE under reducing condition)

purified by

immobilized metal affinity chromatography (IMAC)

species reactivity

human

concentration

1 mg/mL

technique(s)

ELISA: 1:1000-1:25000
immunofluorescence: 1:200-1:600
immunohistochemistry: 1:200-1:600

color

colorless

pH

( in PBS, pH 7.4)

isotype

VHH

Protein ID accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

General description

EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and erbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis. EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2/neu, ErbB-3/HER-3/neu and ErbB-4/HER-4neu. EGFR can heterodimerize with each of the members of this family.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.

Specificity

Human Epidermal Growth Factor Receptor 1 (HER1/EGFR)

Immunogen

Recombinant human EGFR (Leu25-Ser645) protein

Application

ELISA, IHC,IF

Packaging

50 µg per vial / 100 µg per vial

Physical form

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.9

Reconstitution

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage and Stability

Stable for 1 year at 4°C from date of receipt.

Other Notes

Storage at 4°C; Do not freeze.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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