SAB4200717

Anti-Troponin T antibody, Mouse monoclonal

clone JLT-12, purified from hybridoma cell culture

• Synonym(s): Anti-TNNT1, Anti-TNNT2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: JLT-12, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~38 kDa
• species reactivity: rat, human, mouse, bovine, rabbit, goat
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 0.5-1 μg/mL using rat skeletal muscle extract; immunofluorescence: suitable; immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human skeletal muscle sections
• isotype: IgG1
• UniProt accession no.: P13805
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TNNT1(7138)

Description

General description

Anti-Troponin T antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the JLT-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified Troponin T from rabbit skeletal muscle. Troponin T (TNNT) is the tropomyosin binding subunit of the troponin complex. Three genes have evolved to encode the cardiac (TNNT2), slow (TNNT1) and fast (TNNT3) skeletal muscle Troponin T proteins, which are specifically expressed in cardial, slow and fast skeletal muscles, respectively.

Immunogen

purified Troponin T from rabbit skeletal muscle

Application

• Anti-Troponin T antibody, Mouse monoclonal has been used in:
• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

Troponin T (TNNT) participates in calcium regulation of actin thin filament function and is required for the contraction of striated muscles. Levels of cardiac Troponin T serve as a sensitive biomarker of myocardial injury. Troponin T has also been suggested as a potential marker in diagnosis of skeletal myopathies including amyotrophic lateral sclerosis (ALS).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable