R1028
Restorase® DNA Polymerase with 10× Reaction Buffer
Enzyme blend for PCR amplification of damaged DNA
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.55
Recommended Products
Quality Level
form
liquid
usage
sufficient for 50 reactions
feature
Long & Accurate PCR
dNTPs included: no
hotstart: no
concentration
2.5 units/μL
technique(s)
PCR: suitable
color
colorless
input
purified DNA
shipped in
wet ice
storage temp.
−20°C
General description
Restorase DNA Polymerase with 10× Reaction Buffer combines Sigma′s long and accurate enzyme technology with a small amount of DNA repair enzyme. The optimized blend will initiate the repair and further amplification of damaged DNA templates greater than 800 bp. Restorase has also been shown to increase yield on undamaged DNA templates.
Application
Restorase® DNA Polymerase with 10× Reaction Buffer has been used in DNA repair.
Biochem/physiol Actions
DNA templates are often damaged on exposure to heat, acids, alkylating agents, and light. These damaged templates result in inefficient or failed DNA amplification by polymerase chain reaction (PCR). Restorase® DNA Polymerase repairs the damaged sites on the DNA templates and initiates subsequent template copying. Restorase treatment of the DNA depends on the level of template damage.
Features and Benefits
- Reliable amplification of damaged DNA
- When thermostable polymerases fail, this enzyme amplifies the sequence efficiently
- Efficient amplification of sequences in multiplex reactions
- Increased yield and amplicon specificity
- Amplifies a broad spectrum of amplicon sizes varying from 200 bp to 20 kb
- Can repair 3′ bungs, nicks, and abasic sites
Packaging
The enzyme is provided with an optimized 10× reaction buffer provided as 1 vial/250 units.
Other Notes
Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.
View more detailed information on Restorase DNA Polymerase at www.sigma-aldrich.com/restorase.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Restorase is a registered trademark of Merck KGaA, Darmstadt, Germany
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Shigeo Yagi et al.
Parasitology research, 102(2), 211-217 (2007-09-28)
Granulomatous amoebic encephalitis (GAE) is a usually fatal disease caused by the free-living amoebae Balamuthia mandrillaris and Acanthamoeba spp. The intent of this study was to determine if the polymerase chain reaction (PCR) could be used retrospectively to detect amoeba
Francesca Zinetti et al.
PloS one, 8(6), e65746-e65746 (2013-06-12)
There is increasing evidence that most parapatric cryptic/sister taxa are reproductively compatible across their areas of contact. Consequently, the biological species concept, which assumes absence of interbreeding, is becoming a not so effective criterion in evolutionary ecology. Nevertheless, the few
Yuxuan Liu et al.
International journal of legal medicine, 132(3), 675-681 (2017-09-01)
Formalin fixation is considered an important process for preservation of human tissue samples for long periods. However, this process not only results in cross-linking complicating isolation of nucleic acid but also introduces polymerase "blocks" during polymerase chain reaction (PCR). At
James M Robertson et al.
Forensic science international. Genetics, 12, 168-180 (2014-07-06)
Forensic scientists have used several approaches to obtain short tandem repeat (STR) profiles from compromised DNA samples, including supplementing the polymerase chain reaction (PCR) with enhancers and using procedures yielding reduced-length amplicons. For degraded DNA, the peak intensities of the
Mehrdad Hajibabaei et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 360(1462), 1959-1967 (2005-10-11)
Large-scale DNA barcoding projects are now moving toward activation while the creation of a comprehensive barcode library for eukaryotes will ultimately require the acquisition of some 100 million barcodes. To satisfy this need, analytical facilities must adopt protocols that can
Articles
Restorase® was developed for researchers unable to achieve amplification of damaged DNA templates when using other commercially available DNA polymerases.
Protocols
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
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