P5188

TWEEN^® 80

for molecular biology, syrup

• Synonym(s): POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80
• CAS Number: 9005-65-6
• MDL number: MFCD00082107
• UNSPSC Code: 12161900
• NACRES: NA.21

Properties

• grade: for molecular biology
• description: non-ionic
• form: syrup
• mol wt: average mol wt 1310
• composition: Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)
• CMC: 0.012 mM
• transition temp: cloud point 65 °C
• density: 1.06 g/mL at 25 °C
• HLB: 15
• suitability: molecular biology tested
• foreign activity: DNAse (Exonuclease Detection), NICKase (Endonuclease Detection), RNAse, none detected
• SMILES string: CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO
• InChI: 1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+
• InChI key: RGPBUVUVZKQNHD-MDZDMXLPSA-N

Description

General description

TWEEN 80 is a polyethylene sorbitol ester and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): >235.4 °F - closed cup
• Flash Point(C): > 113 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves