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MAK115

Sigma-Aldrich

Hemoglobin Assay Kit

sufficient for 250 colorimetric tests

Synonym(s):

Hemoglobin Quantitation Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 250 colorimetric tests

detection method

colorimetric

relevant disease(s)

hematological disorder

storage temp.

2-8°C

Gene Information

General description

Hemoglobin (Hb) is an iron-containing metalloprotein that serves as the primary means of oxygen transport in vertebrates. Hemoglobin is primarily found in red blood cells where it makes up to 97% of the cell′s dry content. Hemoglobin can also be found in other tissues where it serves as an antioxidant. Alterations in blood hemoglobin levels occurs in many diseases such as anemia and polycytheima.

Application

Hemoglobin Assay Kit has been used to measure the concentration of hemoglobin.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of hemoglobinin a variety of samples such as blood, serum, plasma, and urine. This kit can be used to study the effects of various compounds on hemoglobin metabolism and levels.

Principle

This assay is based on the improved Triton[NP1]/NaOH method in which hemoglobin is converted to a colorimetric product measured at 400 nm. This assay has a linear detection range between 0.9-200 mg/dL in the 96 well plate assay.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Pictograms

CorrosionEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Daniel F Gomez Isaza et al.
Conservation physiology, 8(1), coz092-coz092 (2020-01-29)
Human activities present aquatic species with numerous of environmental challenges, including excessive nutrient pollution (nitrate) and altered pH regimes (freshwater acidification). In isolation, elevated nitrate and acidic pH can lower the blood oxygen-carrying capacity of aquatic species and cause corresponding
Essie M Rodgers et al.
The Journal of experimental biology, 220(Pt 19), 3556-3564 (2017-10-06)
Survival of air-breathing, diving ectotherms is dependent on their capacity to optimise the time available for obligate underwater activities, such as predator avoidance. Submergence times are thermally sensitive, with dive durations significantly reduced by increases in water temperature, deeming these
Baris R Mutlu et al.
Scientific reports, 7(1), 9915-9915 (2017-09-01)
Microfluidic blood processing is used in a range of applications from cancer therapeutics to infectious disease diagnostics. As these applications are being translated to clinical use, processing larger volumes of blood in shorter timescales with high-reliability and robustness is becoming
Mechanisms linking red blood cell disorders and cardiovascular diseases.
Mozos I, et al.
BioMed Research International, 2015 (2015)
Benefits of fiber and antioxidant power of rice bran in Vitamin E deficient rats.
Carlos A G, et al.
Acta Bioquimica Clinica Latinoamericana, 51(1), 95-105 (2017)

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