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M5550

Sigma-Aldrich

MEM Amino Acids (50x) solution

Without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

MEM Amino Acids mixture

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About This Item

MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
NACRES:
NA.75
Pricing and availability is not currently available.

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

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Application

Adding supplements of amino acids to media stimulates growth and prolongs the viability of cells in culture. MEM Amino Acids (50x) solution concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro. It has been used in in vitro cultures,[1] co-cultures[2] and incubation media.[3]

Storage and Stability

This product may contain a yellow hue if exposed to light. This is due to the presence of the amino acids tryosine and tryptophan in the solution. The change in color to the liquid solution has no affect to the quality or integrity of the product. For a complete list of components, please see the formulation sheet listed in the "Documents" section of the product page.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Questions

1–5 of 5 Questions  
  1. How long can this be stored in the refrigerator for?

    1 answer
    1. This product is not assigned an expiration date. Products that have no expiration date are robust in nature and extremely stable in the original sealed container. These products will have no dating reported on either the label or the Certificate of Analysis but are covered by a warranty period of 1 year from the date of product shipment. The complete Product Dating statement may be reviewed at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/190/408/product-dating-information-ms.pdf .

      Helpful?

  2. What are the concentrations of AAs?

    1 answer
    1. The concentration of amino acids can be found in the formulation documentation, please view the formulation document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/210/498/m5550for.pdf.

      Helpful?

  3. Can this be used for cell free expression on E.coli system?

    1 answer
    1. This product is intended for use in mammalian cell culture, not for bacteria.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Can Product M5550, MEM Amino Acids (50X) solution contain precipitates?

    1 answer
    1. Product M5550, MEM Amino Acids (50X) has a specification as a clear solution.  This product can have a slight precipitate that forms at 2-8°C.  This precipitate will disappear when warmed to room temperature or when diluted to 1X.  There is no affect on the product quality.

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