Please check the product information sheet for F4375 which has a detailed protocol.
F2637
Ficoll® 400
BioXtra, lyophilized powder
Synonym(s):
Polysucrose 400, Ficoll 400
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About This Item
Pricing and availability is not currently available.
biological source
non-animal source
Quality Segment
grade
Molecular Biology
product line
BioXtra
form
lyophilized powder
foreign activity
DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
storage temp.
room temp
General description
Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.
Application
Suitable for
- hybridization experiments to reduce non-specific binding of labeled probes to the membrane[1]
- preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
- separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
- producing density gradients to separate subcellular components
- vitrification of cell and tissue samples.[2]
- to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).[3]
Used for cell separation and organelle isolation.
Features and Benefits
- free from DNases, RNases, phosphatases and proteases
- preserves functional and morphological integrity of the separated cells
Other Notes
A nonionic synthetic polymer of sucrose.
Legal Information
Ficoll is a registered trademark of Cytiva
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This Item | |||
|---|---|---|---|
| grade Molecular Biology | grade - | grade - | grade - |
| biological source non-animal source | biological source - | biological source - | biological source sugar beets (Beta vulgaris) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form - | form lyophilized powder |
| storage temp. room temp | storage temp. 15-25°C | storage temp. - | storage temp. 15-25°C |
| product line BioXtra | product line BioXtra | product line BioReagent | product line - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Do you have a protocols for use of Product F2637, Ficoll, in various applications?
1 answer-
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Is Product F2637, Ficoll, the same as Ficoll 400?
1 answer-
Yes. It is Ficoll 400. This product is graded molecular biology grade and is free of any nucleases
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Can you use Product F2637, Ficoll, to isolate mononuclear cells?
1 answer-
When trying to isolate mononuclear cells you can use this product but we offer Histopaque that is a mixture of Ficoll and Hypaque. For humans, we offer H8889, Histopaque®-1077 Hybri-Max™ or 1077-1, Histopaque®-1077. For rodents we offer 1083-1, Histopaque®-1083. For isolation of neutrophils, we offer 1119-1, Histopaque®-1119.
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What is the difference between Ficoll 400 and Ficoll PM 400?
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The difference between Ficoll 400 and Ficoll PM 400 is the PM 400 has stricter specifications on the number of microorganisms/g and the endotoxin level is < 0.125 EU/ml
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product F2637, Ficoll, be used as a component of a DNA loading buffer?
1 answer-
This product can be used in a DNA loading buffer with the following recipe to prepare a 6X stock:0.25% bromophenol blue0.25% xylene cyanol FF15% Ficoll
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