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ECM002

Sigma-Aldrich

Thrombospondin-1 human

recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Synonym(s):

THBS, THBS1, TSP, TSP1, Thrombospondin-1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

sterility

sterile

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

127.5 kDa (The protein migrates as a 140 kDa band on SDS-PAGE due to glycosylation.)

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg endotoxin, tested

solubility

water: soluble

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... THBS1(7057)

General description

Thrombospondin-1 (TSP1) belongs to family of glycoproteins and possesses a complex trimeric structure. The structure comprises a heparin-binding domain and a procollagen homology domain located at the amino terminus, while the carboxyl-terminal end contains type I, II, and III repeats. This protein is manufactured in human cells using an all-human production system, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture. Recombinant human thrombospondin-1 is expressed in HEK293 cells as a glycoprotein with a calculated moleculaer mass of 127.5 kDa.

Application

Thrombospondin 1 human (TSP1) has been used:

  • To treat hepatocyte (IHH) cancer cells to study the role of aspartyl protease 1 (MfSAP1) in extracellular matrix degradation.
  • In in vitro Extracellular Matrix (ECM) protein degradation assays.
  • In rat C6 astroglioma cells, for coating the cell culture plates to study the effects of integrins on CNTF (ciliary neurotrophic factor) expression.
  • In the preparation of secreted factor cocktail for analysis of the human mesenchymal stem cell secretome.
    Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.1-25 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.

Biochem/physiol Actions

Thrombospondin (TSP1) plays a crucial role in guiding extracellular matrix (ECM) synthesis and facilitating dynamic tissue remodeling. It is involved in various biological processes including angiogenesis, matrix assembly, activation of transforming growth factor (TGF), and promotion of neurite outgrowth. TSP1 binds to various cell surface receptors, such as integrins and integrin-associated protein CD47. Studies have also shown that the signaling pathway involving TSP1-CD47 promotes the generation of reactive oxygen species (ROS) in human pulmonary artery endothelial cells. This signaling pathway plays a significant role in the manifestation of sickle cell-associated vasculopathy and the subsequent progression of pulmonary hypertension. Moreover, hypoxia-inducible factor (HIF)-2α has been shown to upregulate the expression of TSP1 under hypoxic conditions. TSP1 also plays a crucial role in inflammatory processes and post-inflammatory tissue dynamics. It is upregulated in rheumatoid synovial tissues and might be associated with rheumatoid arthritis.

Features and Benefits

  • Human-derived thrombospondin
  • Produced through recombinant expression in HEK 293 cells
  • Low endotoxin levels

Physical form

Supplied as a powder, lyophilized from phosphate buffered saline.

Analysis Note

The biological activity of recombinant human thrombospondin-1 was tested in culture by measuring the ability of immobilized DTT-treated thrombospondin-1 to support adhesion of SVEC4-10 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Functions of Thrombospondin-1 in the Tumor Microenvironment
Kaur S, et al.
International Journal of Molecular Sciences, 22(9), 4570-4570 (2021)
Thrombospondin-1 CD47 Signalling: From Mechanisms to Medicine
Kale A, et al.
International Journal of Molecular Sciences, 22(8), 4062-4062 (2021)
Identification of Malassezia furfur Secreted Aspartyl Protease 1 (MfSAP1) and Its Role in Extracellular Matrix Degradation
Si En Poh, et al.
Frontiers in Cellular and Infection Microbiology, 10 (2020)
Artificial Extracellular Matrices to Functionalize Biomaterial Surfaces
Bierbaum S, et al.
Comprehensive Biomaterials, 2 (2017)
Thrombospondin-1 and Transforming Growth Factor Beta Are Pro-Inflammatory Molecules in Rheumatoid Arthritis
Rico MC, et al.
The Journal of Laboratory and Clinical Medicine, 152(2), 95?98-95?98 (2009)

Articles

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

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