Key Documents

View All Documentation

DMEM - high glucose

Name: DMEM - high glucose
Brand: SIGMA

HEPES modification, with sodium bicarbonate, without ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

Select a Size

Change View

Size/SKU	Availability	Price

About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

Dulbecco′s Modified Eagle′s Medium - high glucose, HEPES modification, With 4500 mg/L glucose, 25 mM HEPES, and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
HEPES: 25 mM
glucose: high
NaHCO₃: yes
L-glutamine: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

Listen to an Audio Overview

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Supplement with 0.584 g/L L-glutamine.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	D1145	D0422	D1152
SAFC D6171 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D1145 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D0422 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D1152 Dulbecco′s Modified Eagle′s Medium - high glucose
description HEPES modification, With 4500 mg/L glucose, 25 mM HEPES, and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture	description With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture	description With 4500 mg/L glucose and sodium bicarbonate, without L-methionine, L-cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture	description HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility -
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form liquid	form powder
components sodium pyruvate: no HEPES: 25 mM glucose: high NaHCO₃: yes L-glutamine: no phenol red: yes	components L-glutamine: no sodium pyruvate: no glucose: high phenol red: no NaHCO₃: yes HEPES: no	components glucose: high phenol red: yes L-glutamine: no sodium pyruvate: yes	components sodium pyruvate: no NaHCO₃: no phenol red: yes HEPES: 25 mM L-glutamine: yes glucose: high
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

Still not finding the right product?

Explore all of our products under Dulbecco′s Modified Eagle′s Medium - high glucose

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

Rating Snapshot

Select a row below to filter reviews.

Average Customer Ratings

Overall, average rating value is 5 of 5.

Active Filters

1 Ratings-only review