C5585

Monoclonal Anti-Biotin–Cy3^™ antibody produced in mouse

clone BN-34, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-Biotin
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: CY3 conjugate
• Clone: BN-34, monoclonal
• Application: IF, IHC (p)
• Citations: 7

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: CY3 conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BN-34, monoclonal
• form: buffered aqueous solution
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, indirect immunofluorescence: 1:200
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the BN-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Biotin is a naturally occurring vitamin and co-enzyme required by cells in living organisms or in culture.

The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

Immunogen

Biotin-KLH conjugate

Application

May be substituted for avidin to decrease non-specific binding.

Monoclonal Anti-Biotin−Cy3^™ antibody produced in mouse has been used in:

• fluorescent microscopy
• RNA fluorescence in situ hybridization (FISH){36
• immunostaining

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Biochem/physiol Actions

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.

Monoclonal Anti-Biotin- Cy3 can be used in many other applications where biotin can be introduced as target label. It can be used in detection of DNA and mRNA by sensitive non-isotopic in situ hybridization. It may also be used for detection of microinjected biotin-haptenized cytoskeletal proteins allowing examination of the pattern of incorporation and turnover of cytoskeletal proteins in living cells.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)