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Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12

Name: Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12
Brand: SIGMA

liquid, suitable for cell culture

Synonym(s):

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DME/F-12, 1:1 mixture

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217417

Pricing and availability is not currently available.

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Properties

Quality Segment

500

description

for research or for further manufacturing use, with 3151 mg/L dextrose, with 2.5 mM L-glutamine, with 15mM HEPES, with 55 mg/L sodium pyruvate

sterility

sterile

form

liquid

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.055 g/L
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

DMEM/F12 was originally developed to study hormonal requirements of cells in culture.

Application

Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 has been used as a culture media for mouse muscle myoblast C2C12 and human bone marrow cell line SH-SY5Y.

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This Item	51448C	D8900	D6434
SAFC 51445C Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12	SAFC 51448C Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12	Sigma-Aldrich D8900 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D6434 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
description liquid, suitable for cell culture	description suitable for cell culture	description With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture	description With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
sterility sterile	sterility sterile	sterility -	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| stem cell: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form powder	form liquid
components sodium pyruvate: 0.055 g/L phenol red: yes glucose: 3.15 g/L L-glutamine: yes	components phenol red: yes L-glutamine: yes sodium pyruvate: 0.055 g/L glucose: 3.15 g/L	components sodium pyruvate: 0.055 g/L HEPES: 15 mM NaHCO₃: no L-glutamine: yes glucose: 3.15 g/L phenol red: yes	components L-glutamine: no phenol red: no NaHCO₃: yes HEPES: 15 mM sodium pyruvate: 0.055 g/L glucose: 3.15 g/L
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

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Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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