38922
Abberior® STAR 512, NHS ester
for STED application
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
Quality Level
Assay
≥80.0% (degree of coupling)
mol wt
Mw 836.7 g/mol
solubility
DMF: 0.25 mg/mL, clear
fluorescence
λex 510 nm; λem 530 nm±5 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Absorption Maximum, λmax: 517 nm (MeOH),
511 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 85,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.24 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.07 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 533 nm (MeOH),
530 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 −620 nm
Fluorescence Quantum Yield, η: 0.82 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 4.1 ns (PBS, pH 7.4)
511 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 85,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.24 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.07 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 533 nm (MeOH),
530 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 −620 nm
Fluorescence Quantum Yield, η: 0.82 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 4.1 ns (PBS, pH 7.4)
Application
Abberior® Star 512 labelled phosphoethanolamine lipid analogues were used for gated STED-FCS (stimulated emission depletion - fluorescence correlation spectroscopy) study.
Suitability
Designed and tested for fluorescent super-resolution microscopy
Other Notes
Legal Information
abberior is a registered trademark of Abberior GmbH
related product
Product No.
Description
Pricing
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Mathias P Clausen et al.
Methods (San Diego, Calif.), 88, 67-75 (2015-07-01)
Recent years have seen the development of multiple technologies to investigate, with great spatial and temporal resolution, the dynamics of lipids in cellular and model membranes. One of these approaches is the combination of far-field super-resolution stimulated-emission-depletion (STED) microscopy with
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported
Volker Westphal et al.
Physical review letters, 94(14), 143903-143903 (2005-05-21)
Utilizing single fluorescent molecules as probes, we prove the ability of a far-field microscope to attain spatial resolution down to 16 nm in the focal plane, corresponding to about 1/50 of the employed wavelength. The optical bandwidth expansion by nearly
Beitraege zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung.
Abbe, E.
Archiv fur Mikroskopische Anatomie, 9, 413-420 (1873)
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