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P0290

Sigma-Aldrich

Phenol red solution

0.5%, liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Phenolsulfonphthalein solution

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

product line

BioReagent

form

liquid

concentration

0.5%

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

SMILES string

O=S1(C2=CC=CC=C2C(C3=CC=C(O)C=C3)(C4=CC=C(O)C=C4)O1)=O

InChI

1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H

InChI key

BELBBZDIHDAJOR-UHFFFAOYSA-N

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General description

Phenol red belongs to the class of triphenylmethane dyes and is widely used in the textile industry.

Application

Phenol red solution has been used:
  • as a tracer dye for microinjection of zebrafish eggs
  • as a component of the mMW medium for the preparation of mouse embryos
  • in the preparation of versene-ethylenediamine tetraacetic acid (EDTA) solution
  • as a pH indicator in Dulbecco′s modified Eagle medium (DMEM) during glycolytic stress (GST) tests and mitochondrial stress tests (MST) to evaluate metabolic function in cells.

Biochem/physiol Actions

Phenol red solution acts as a pH indicator for the culture medium.Phenol red solution acts as a pH indicator for the culture medium that turns yellow at a pH of 6.4 or lower and changes into red at a pH of 8.2 or higher. It enables a fast and simple assessment of the culture′s health. In addition to its application as a pH indicator in culture mediums, Phenol red is used to detect human peroxidase enzyme activity, as well as cellular hydrogen peroxide levels, in various experiments.

Features and Benefits

High-quality, endotoxin-tested solution, suitable for mammalian cell culture.

Preparation Note

Prepared in Dulbecco′s Phosphate Buffered Saline.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Shivshankar R Mane et al.
Small (Weinheim an der Bergstrasse, Germany), 14(36), e1801571-e1801571 (2018-08-07)
Recent advances in super-resolution microscopy and fluorescence bioimaging allow exploring previously inaccessible biological processes. To this end, there is a need for novel fluorescent probes with specific features in size, photophysical properties, colloidal and optical stabilities, as well as biocompatibility
Natacha Merindol et al.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 128, 104423-104423 (2020-05-18)
Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR
Tina Cheng et al.
Micron (Oxford, England : 1993), 129, 102782-102782 (2019-11-28)
Infection of zebrafish with natural pathogen Mycobacterium marinum is a useful surrogate for studying the human granulomatous inflammatory response to infection by Mycobacterium tuberculosis. The adaptive immune system of the adult stage zebrafish offers an advance on the commonly used
Il Hwan Kim et al.
Cell reports, 32(4), 107965-107965 (2020-07-30)
Psychiatric disorders are highly heritable pathologies of altered neural circuit functioning. How genetic mutations lead to specific neural circuit abnormalities underlying behavioral disruptions, however, remains unclear. Using circuit-selective transgenic tools and a mouse model of maladaptive social behavior (ArpC3 mutant)
Pawel Kordowitzki et al.
Aging, 12(8), 7576-7584 (2020-04-28)
Maternal aging-associated reduction of oocyte viability is a common feature in mammals, but more research is needed to counteract this process. In women, the first aging phenotype appears with a decline in reproductive function, and the follicle number gradually decreases

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