U0508

Monoclonal Anti-Ubiquitin antibody produced in mouse

clone 6C1, ascites fluid

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03792436
• Conjugate: unconjugated
• Clone: 6C1, monoclonal
• Application: ARR, ELISA (i), WB
• Citations: 37

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 6C1, monoclonal
• contains: 15 mM sodium azide
• species reactivity: human, rat, mouse, bovine
• technique(s): indirect ELISA: suitable, microarray: suitable, western blot: 1:1,000 using whole cell lysate of human promyelocitic HL60 cells
• isotype: IgG2a
• UniProt accession no.: P0CG47
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... UBB(7314), UBC(7316) ; mouse ... Ubb(22187), Ubc(22190) ; rat ... Ubb(192255), Ubc(50522)

Description

General description

Ubiquitin, an 8.5kD (approx.) 76 amino acid containing protein, is highly conserved and found in both prokaryotes and eukaryotes. It facilitates protein degradation in ubiquitin/proteasome pathway and also has pivotal role in intracellular regulation. Monoclonal anti-ubiquitin antibody can be used in immunoblotting and ELISA to localize and detect ubiquitin. Mouse anti-ubiquitin antibody reacts specifically with ubiquitin in mouse, human, bovine and rat.

Immunogen

purified bovine ubiquitin conjugated to KLH.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal anti-ubiquitin antibody can be used in western blot to visualize and evaluate ubiquitylated proteins.It can also be used in microarray and immunoblotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable