If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed. This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.
TOX6
In Vitro Toxicology Assay Kit, Sulforhodamine B based
Synonym(s):
biomass viability assay, sulforhodamine B, total biomass assay
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About This Item
NACRES:
NA.84
UNSPSC Code:
12352207
Pricing and availability is not currently available.
form
liquid
Quality Segment
usage
kit sufficient for 1,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
565 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
room temp
General description
The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.
Application
In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.
Biochem/physiol Actions
Dye binds to cellular protein and is then solubilized in base.
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This Item | |||
|---|---|---|---|
| description - | description - | description - | description - |
| storage condition dry at room temperature | storage condition dry at room temperature | storage condition dry at room temperature | storage condition dry at room temperature |
| application(s) cell analysis | application(s) cell analysis | application(s) cell analysis | application(s) cell analysis |
| detection method colorimetric | detection method colorimetric, fluorometric | detection method colorimetric | detection method colorimetric |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. room temp | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
signalword
Danger
wgk
WGK 2
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
Storage Class
8A - Combustible corrosive hazardous materials
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When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?
1 answer-
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?
1 answer-
Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture.The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension. Otherwise, you need to centrifuge, remove the media and then fix. Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003.
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