T9326

Thrombin human

BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE)

• Synonym(s): Factor IIa
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.4.21.5 (BRENDA, IUBMB)
• MDL number: MFCD00082072
• Specific activity: ≥2,000 units/mg protein
• Assay: ≥95% (SDS-PAGE)
• Recombinant: expressed in HEK 293 cells

Properties

• recombinant: expressed in HEK 293 cells
• Quality Segment: 200
• product line: BioUltra
• assay: ≥95% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥2,000 units/mg protein
• mol wt: 37.4 kDa
• UniProt accession no.: P00734
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... F2(2147)

Description

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess in vitro hemostatic properties of French lyophilized plasma.

Physical form

supplied as a solution in 20 mM MES, pH 6.0, 500 mM choline chloride

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable