T8877 is tested for solubility at 50 mg/ml in water. Although no suitability testing is performed, 2,3,5-Triphenyltetrazolium chloride (Tetrazolium Red) is often used to assess plant viability using a 10 mg/ml solution. Other uses, such as staining brain or heart tissue, have been reported.
T8877
2,3,5-Triphenyltetrazolium chloride
≥98.0% (HPLC)
2,3,5-Triphenyltetrazolium chloride
1 of 2 reviewers received a sample product or took part in a promotion
Synonym(s):
TPTZ, TTZ, Tetrazolium Red
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About This Item
Empirical Formula (Hill Notation):
C19H15ClN4
CAS Number:
Molecular Weight:
334.80
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
206-071-6
Beilstein/REAXYS Number:
3923356
MDL number:
Pricing and availability is not currently available.
Quality Segment
assay
≥98.0% (HPLC)
form
powder
color
faintly yellow
solubility
H2O: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4
InChI
1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1
InChI key
PKDBCJSWQUOKDO-UHFFFAOYSA-M
General description
2,3,5-Triphenyltetrazolium chloride is a colorless water-soluble dye. In the mitochondria of living cells, it is reduced to a deep red, water-insoluble compound (formazan). 2,3,5-Triphenyltetrazolium chloride helps to distinguish between viable and infarcted brain tissue after stroke.
Application
2,3,5-Triphenyltetrazolium chloride has been used to stain heart tissue to measure the extent of acute lesion. It has also been used to stain brain tissue to check the size of infarct area.
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This Item | |||
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C |
| solubility H2O: 50 mg/mL | solubility acetonitrile: 50 mg/mL | solubility chloroform: 1 mg/mL, clear, red | solubility water: 10 mg/mL, dark red |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| assay ≥98.0% (HPLC) | assay 97% | assay - | assay - |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2
Storage Class
4.1B - Flammable solid hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Hello, I wasn’t able to find the recommended proportion of product to water. Could you let me know what ratio I should use? Also, should I be using distilled water? Thank you!
1 answer-
Helpful?
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Bonjour, après avoir reconstitué la poudre dans de l'eau stérile, combien de temps pouvons-nous conserver la préparation au réfrigérateur ? Merci
1 answer-
The long-term solution stability of this product has not been determined. However, product 17779, a sterile 1% solution, has a shelf life of approximately 3 years. Provided that the solution is filter sterilized and stored properly at 2-8°C, a similar shelf life would be expected. Please see the link below to review this product:
https://www.sigmaaldrich.com/product/sial/17779Helpful?
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