T3411

Tetramethylammonium chloride solution

Molecular Biology

• Synonym(s): N,N,N-Trimethylmethanaminium chloride
• CAS Number: 75-57-0
• NACRES: NA.31
• PubChem Substance ID: 24900143
• UNSPSC Code: 12352107
• MDL number: MFCD00011628

Properties

• grade: Molecular Biology
• Quality Segment: 200
• concentration: 5 M
• foreign activity: DNase, RNase, none detected
• SMILES string: [Cl-].C[N+](C)(C)C
• InChI: 1S/C4H12N.ClH/c1-5(2,3)4;/h1-4H3;1H/q+1;/p-1
• InChI key: OKIZCWYLBDKLSU-UHFFFAOYSA-M

Description

General description

Tetramethylammonium binds AT-rich DNA polymers while concomitantly abolishing the preferential melting of AT versus GC base pairs. It is supplied as a 0.2 μm filtered solution in 18 megohm water.

Application

Tetramethylammonium chloride solution (TMAC) has been used:

• in the preparation of hybridization cocktail for array hybridization and scanning
• in next-generation sequencing (NGS), and genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) library preparation
• in the preparation of TMAC buffer and bead hybridization mixture for hybridization and detection