T3285
TNM-FH insect medium
With ʟ-glutamine and sodium bicarbonate,liquid, sterile-filtered, suitable for insect cell culture
Synonym(s):
supplemented Grace′s medium
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About This Item
Product Name
TNM-FH insect medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | insect: suitable
components
NaHCO3: 0.35 g/L
L-glutamine: 0.6 g/L
shipped in
ambient
storage temp.
2-8°C
General description
Several modifications of Grace′s medium were developed in several laboratories in the late 1960′s and 1970′s. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species.
Application
TNM-FH insect medium has been used to culture Sf9 insect cells.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Thomas Piechulek et al.
The Journal of biological chemistry, 280(47), 38923-38931 (2005-09-21)
The regulation of the two isoforms of phospholipase C-gamma, PLCgamma(1) and PLCgamma(2), by cell surface receptors involves protein tyrosine phosphorylation as well as interaction with adapter proteins and phosphatidylinositol 3,4,5-trisphosphate (PtdInsP(3)) generated by inositol phospholipid 3-kinases (PI3Ks). All three processes
J J Gallardo-Rodríguez et al.
Bioresource technology, 200, 699-705 (2015-11-12)
A modification of a flow contraction device was used to subject shear-sensitive microalgae to well-defined hydrodynamic forces. The aim of the study was to elucidate if the inhibition of shear-induced growth commonly observed in dinoflagellate microalgae is in effect due
Effects of media constituents on growth of insect cells in stationary and suspension cultures.
Hink, W.F., et al.
In Vitro, 9, 371-371 (1974)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T3285-10L | 04061837036798 |
| T3285-500ML | 04061837344084 |
| T3285-6X1L | 04061837344091 |
| T3285-6X500ML | 04061837344107 |
| T3285-1L | 04061837344077 |