T2507

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

• Synonym(s): D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
• CAS Number: 9023-78-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-774-2
• MDL number: MFCD00082080
• EC Number: 5.3.1.1 (BRENDA, IUBMB)
• Specific activity: ~10,000 units/mg protein

Properties

• type: Type I
• Quality Segment: 200
• form: ammonium sulfate suspension
• specific activity: ~10,000 units/mg protein
• mol wt: 53 kDa by equilibrium centrifugation
• foreign activity: pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%
• storage temp.: 2-8°C

Description

Application

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

Physical form

Crystalline suspension in 2.7 M (NH₄)₂SO₄, 0.5 mM EDTA, pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)