T1949
Total Protein Reagent
Synonym(s):
Biuret Reagent
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About This Item
NACRES:
NC.07
UNSPSC Code:
12352202
Quality Segment
Application
Total Protein Reagent has been used in the biuret assay.
Total Protein Reagent can be used for the quantitative, colorimetric determination of total protein concentration in solution at 540 nm. The total protein reagent is supplied as a ready-to-use liquid.
Biochem/physiol Actions
A biuret-based total protein reagent. Copper ions in the alkaline reagent react with peptide bonds in proteins resulting in a purple color with an absorbance maximum of 540 nm.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Related Content
Instructions
Determination of serum proteins by means of the biuret reagent.
Gornall, A. G., et al.,
The Journal of Biological Chemistry, 177, 759-759 (1949)
B T Doumas et al.
Clinical chemistry, 27(10), 1642-1650 (1981-10-01)
We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) and Standard Reference Material (SRM) 927 bovine albumin (National Bureau
Systematic comparisons of various spectrophotometric and colorimetric methods to measure concentrations of protein, peptide and amino acid: detectable limits, linear dynamic ranges, interferences, practicality and unit costs.
Chutipongtanate S
Talanta, 98, 123-123 (2012)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T1949-500ML | 04061837338458 |