SML2416

HET0016

≥95% (HPLC)

• Synonym(s): HET 0016, HET-0016, N′-(4-Butyl-2-methylphenyl)-N-hydroxymethanimidamide, N-(4-Butyl-2-methylphenyl)-N′-hydroxyformamidine, N-(4-Butyl-2-methylphenyl)-N′-hydroxymethanimidamide, N-Hydroxy-N′-(4-n-butyl-2-methylphenyl)formamidine
• Empirical Formula (Hill Notation): C₁₂H₁₈N₂O
• CAS Number: 339068-25-6
• Molecular Weight: 206.28
• NACRES: NA.77
• UNSPSC Code: 12352200
• Assay: ≥95% (HPLC)
• Form: powder
• Storage condition: desiccated

Properties

• assay: ≥95% (HPLC)
• form: powder
• storage condition: desiccated
• color: white to beige
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: −20°C
• SMILES string: CCCCC1=CC=C(C(C)=C1)/N=C/NO
• InChI: 1S/C12H18N2O/c1-3-4-5-11-6-7-12(10(2)8-11)13-9-14-15/h6-9,15H,3-5H2,1-2H3,(H,13,14)
• InChI key: LYNOGBKNFIHKLE-UHFFFAOYSA-N

Description

Biochem/physiol Actions

HET0016 is a potent and selective inhibitor against cytochrome P450 (CYP450) ω-hydroxylases (human CYP4A11/4A22/4F2/4F3, rat CYP4A1/4A2/4A3/4A8)-mediated 20-hydroxyeicosatetraenoic acid (20-HETE) biosynthesis (IC50 = 8.9/35 nM using human/rat renal microsomes), exhibiting much reduced potency against epoxyeicosatrienoic acids (EETs) biosynthesis (IC50 = 2.8 μM/rat microsome) or the enzyme activities of CYP2C9/2D6/3A4 and COX (IC50 = 3.3/83.9/71 μM and 2.3 μM). HET0016 is widely employed both in cultures (1-10 μM) and in aminals in vivo (1-10 mg/kg via i.v., i.m., i.p. or s.c.) for probing 20-HETE-dependent physiological and pathological processes.

Potent and selective inhibitor against cytochrome P450 (CYP450) ω-hydroxylases-mediated 20-hydroxyeicosatetraenoic acid (20-HETE) biosynthesis.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable