SAE0104

BmrA (Bacilus Subtilis Multidrug Resistance Abc Transporter Atp-Binding Protein)

• NACRES: NA.83
• UNSPSC Code: 12352202
• Form: aqueous solution
• Assay: ≥90% (SDS-PAGE)
• Recombinant: expressed in E. coli
• Mol wt: 64.5 kDa

Properties

• recombinant: expressed in E. coli
• description: C-Terminal six-histidine tagged
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 64.5 kDa
• concentration: 0.3 mg/mL
• UniProt accession no.: O06967
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

MSSSHHHHHHMPTKKQKSKSKLKPFFALVRRTNPSYGKLAFALALSVVTTLVSLLIPLLTKQLVDGFSMSNLSGTQIGLIALVFFVQAGLSAYATYALNYNGQKIISGLRELLWKKLIKLPVSYFDTNASGETVSRVTNDTMVVKELITTHISGFITGIISVIGSLTILFIMNWKLTLLVLVVVPLAALILVPIGRKMFSISRETQDETARFTGLLNQILPEIRLVKASNAEDVEYGRGKMGISSLFKLGVREAKVQSLVGPLISLVLMAALVAVIGYGGMQVSSGELTAGALVAFILYLFQIIMPMGQITTFFTQLQKSIGATERMIEILAEEEEDTVTGKQIENAHLPIQLDRVSFGYKPDQLILKEVSAVIEAGKVTAIVGPSGGGKTTLFKLLERFYSPTAGTIRLGDEPVDTYSLESWREHIGYVSQESPLMSGTIRENICYGLERDVTDAEIEKAAEMAYALNFIKELPNQFDTEVGERGIMLSGGQRQRIAIARALLRNPSILMLDEATSSLDSQSEKSVQQALEVLMEGRTTIVIAHRLSTVVDADQLLFVEKGEITGRGTHHELMASHGLYRDFAEQQLKMNADLENKAG

Biochem/physiol Actions

BmrA is an efflux protein and ABC transporter that acts as an ATP-dependent drug efflux pump.

Preparation Note

Formulated in 50mM Tris-Cl pH 8.0, 100mM NaCl, 0.01% DDM, 10% glycerol.

Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot into single use aliquots. Store remaining undiluted protein in aliquots at -80°C. Note: avoid freeze/thaw cycles.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable