SAB4504331

Anti-phospho-Akt (pSer⁴⁷³) antibody produced in rabbit

affinity isolated antibody

• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA, IHC, WB
• Citations: 13

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 55 kDa
• species reactivity: human, mouse, rat
• concentration: ~1 mg/mL
• technique(s): ELISA: 1:5000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000
• NCBI accession no.: P31749, P31751, Q9Y243
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer473)
• Gene Information: human ... AKT1(207) ; rat ... Akt1(24185)

Description

General description

AKT1 (AKT serine/threonine kinase 1) is also called as v-akt murine thymoma viral oncogene homolog 1 and PKBα (protein kinase B). It is expressed in liver, muscle and adipocytes. AKT1 gene is mapped to human chromosome 14q32. It is a member of AKT family that has an amino-terminal pleckstrin homology (PH) domain, a short α helical linker and a carboxyl-terminal kinase domain.

Immunogen

The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser473.

Immunogen Range: 431-480

Application

Anti-phospho-Akt (pSer473) antibody has been used in western blotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

AKT1 (AKT serine/threonine kinase 1) controls the survival of cells and anti-apoptotic actions that attack the pathogenesis of several cancers , hence it plays a crucial role in tumorigenesis. In mice, AKT1 is essential for normal development.

Features and Benefits

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Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable