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S8820

Protease Inhibitor Cocktail

SIGMA FAST, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, For General Use

Synonym(s):

Protease Inhibitor Tablets, protease inhibitor

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
Form:
tablet
Solubility:
water: 1 tablet/100mL, clear to hazy, colorless
Storage temp.:
2-8°C
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Product Name

SIGMAFAST™ Protease Inhibitor Tablets, For General Use

Quality Segment

200

biological source

bovine lung, bovine milk

form

tablet

solubility

water: 1 tablet/100mL, clear to hazy, colorless

storage temp.

2-8°C

General description

SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract.
The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products.

Application

SIGMAFAST Protease Inhibitor Tablets has been used:
  • to wash raw semen for membrane protein analysis
  • in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
  • as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
  • as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1β1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)

These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.

Biochem/physiol Actions

Inhibits serine, cysteine, aspartic, and metalloproteases.

Features and Benefits

  • Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
  • Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
  • Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
  • Tested and optimized for general use: proven performance and reliability for a wide range of applications.

Physical form

Tablet that is water soluble.

Preparation Note

One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.

Other Notes

AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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form

tablet

form

tablet

form

powder

form

DMSO solution

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

biological source

bovine lung, bovine milk

biological source

bovine lung

biological source

-

biological source

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

water: 1 tablet/100mL, clear to hazy, colorless

solubility

-

solubility

water: 0.1 g/mL, clear, colorless

solubility

water: soluble

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pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

hcodes

H314,H335

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Questions

1–8 of 8 Questions  

  1. How long does the cocktail keep once a tablet is reconstituted in 10mL of water at 2-8°C?

    1 answer

    1. The reconstituted protease inhibitor solution is stable for at least 2 weeks at 2-8 °C. Please see the datasheet below for more information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/177/562/s8820bul-ms.pdf

      Helpful?

  2. How much of Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, needs to be used in cell lysates?

    1 answer

    1. One tablet is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Can Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?

    1 answer

    1. The tablets contain EDTA, a metal chelator, which may remove metal ions from these affinity products. The suggested protease inhibitor for the HIS-Select products is either Product No. S8830 (water soluble tablet) or P8849 (DMSO solution).

      Helpful?

  5. What is the difference between Protease Inhibitors S8820 and P2714?

    1 answer

    1. S8820 is the tablet version of P2714.

      Helpful?

  6. What is the concentration of the inhibitors in Product S8820, SIGMAFAST™ Protease Inhibitor Tablets?

    1 answer

    1. When reconstituted (one tablet in 100 ml water), concentrations of the component inhibitors are as follows:AEBSF      2 mME-64          14 μMBestatin     130 μMLeupeptin   0.9 μMAprotinin    0.3 μMEDTA        1 mM

      Helpful?

  7. Can Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, be used with ELISA?

    1 answer

    1. Yes, because it is water based.

      Helpful?

  8. How should I dissolve Product S8820, SIGMAFAST™ Protease Inhibitor Tablets?

    1 answer

    1. You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use.

      Helpful?

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