S400HR

Sephacryl^® Size Exclusion Resin

400-HR, MW range 20-8000 kDa (globular proteins)

• Synonym(s): Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)
• CAS Number: 65546-95-4
• UNSPSC Code: 23151817
• NACRES: NA.56
• MDL number: MFCD00165824

Properties

• Product Name: Sephacryl^®, 400-HR, MW range 20-8000 kDa (globular proteins)
• Quality Segment: 200
• description: stability: stable to 0.2M NaOH
• form: suspension (20% aqueous ethanol)
• feature: autoclavable
• matrix: crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
• particle size: 25-75 μm (wet)
• pore size: 10-2000 kDa MW range (dextrans), 20-8000 kDa MW range (globular proteins)
• compatibility: mode of use size exclusion chromatography
• storage temp.: 2-8°C

Description

General description

• Supplied swollen, suspended in 20% aqueous ethanol
• Autoclavable
• Stable to 0.2N sodium hydroxide (NaOH)
• Store at refrigerated temperature (2-8 °C)

S400HR-750ML′s updated product is GE17-0609-01

Application

Sephacryl^® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl^® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl^® has also been used to purify and characterize enzymes such as bovine pancreatic elastase, tissue-nonspecific alkaline phosphatases and a lysozyme from human urine.

For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class: 3 - Flammable liquids
• wgk: WGK 1
• flash_point_f: 100.4 - 109.4 °F - closed cup
• flash_point_c: 38 - 43 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter