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R8000

D-Ribulose 1,5-Diphosphate Carboxylase from spinach

partially purified powder, 0.01-0.1 unit/mg solid

Synonym(s):

3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco

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About This Item

CAS Number:
9027-23-0
EC Number:
4.1.1.39 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00130908
Specific activity:
0.01-0.1 unit/mg solid
Biological source:
spinach
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biological source

spinach

Quality Segment

200

form

partially purified powder

specific activity

0.01-0.1 unit/mg solid

mol wt

557 kDa

storage temp.

−20°C

General description

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.
Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

Application

D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:
  • as a test protein in pepsin digestion studies
  • as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines
  • in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid

Biochem/physiol Actions

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.

Other Notes

One unit will convert 1.0 μmole of D-RuDP and CO2 to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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