R2283

Pepsin Reagent, Ready to Use, Antigen Retriever

suitable for immunohistochemistry

• Synonym(s): pepsin
• NACRES: NA.47
• UNSPSC Code: 12171500

Properties

• biological source: Porcine stomach
• Quality Segment: 100
• application(s): hematology; histology
• storage temp.: 2-8°C

Description

General description

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to the application of antibodies. In immunohistochemistry (IHC), the most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves the accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.

Application

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to the application of antibodies in immunohistochemistry (IHC).It has been used to enhance the permeabilization of fungal cell walls during the fluorescence in situ hybridization (FISH) process and utilized during the RNA scope in situ hybridization process to perform antigen retrieval.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable