P9614

Lambda protein phosphatase

liquid, Bacteriophage Lambda, recombinant, expressed in E. coli

• Synonym(s): Lambda-PPase
• CAS Number: 401941-75-1
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00243301
• Recombinant: expressed in E. coli
• Concentration: ≥400000 units/mL

Properties

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: liquid
• mol wt: 25 kDa
• concentration: ≥400000 units/mL
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Lambda protein phosphatase is a recombinant protein expressed in Escherichia coli. It requires Mn²⁺ or Ni²⁺ as activators.

Biochem/physiol Actions

Can be used to release phosphate groups from serine, threonine or tyrosine residues in proteins. Also active on phosphorylated histidine residues.

Lambda protein phosphatase has been used to dephosphorylate HeLa cell extracts.

Other Notes

One unit will hydrolyze 1 nanomole of p-nitrophenyl phosphate per min at pH 7.5 at 30 deg C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable