P9279
Phospholipase A2 from honey bee venom (Apis mellifera)
salt-free, lyophilized powder, 600-2400 units/mg protein
Synonym(s):
Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-637-7
MDL number:
Specific activity:
600-2400 units/mg protein
Pricing and availability is not currently available.
form
salt-free, lyophilized powder
Quality Segment
specific activity
600-2400 units/mg protein
mol wt
14.5 kDa
UniProt accession no.
storage temp.
−20°C
Gene Information
honey bee ... Pla2(406141)
General description
Phospholipase A2 from honey bee venom (Apis mellifera) is a calcium-dependent lipolytic enzyme.
Application
Phospholipase A2 from honey bee venom (Apis mellifera) has been used
- to determine renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation
- in the treatment of photosystem-II (PSII) dimer complex of Synechocystis to study changes in oxygen-evolving activity
- as a standard in phospholipase activity assay
Biochem/physiol Actions
Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.9 at 25 °C.
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information honey bee ... Pla2(406141) | Gene Information cow ... PLA2G1B(282457) | Gene Information pig ... PLA2G1B(445525) | Gene Information - |
| specific activity 600-2400 units/mg protein | specific activity ≥20 units/mg protein | specific activity ≥600 units/mg protein | specific activity - |
| form salt-free, lyophilized powder | form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| mol wt 14.5 kDa | mol wt - | mol wt - | mol wt ~_2.8 kDa |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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