P9273

Pro-Phe-Arg-7-amido-4-methylcoumarin

≥95%

• Synonym(s): PFR-Nmec
• Empirical Formula (Hill Notation): C₃₀H₃₇N₇O₅ · C₂H₄O₂
• CAS Number: 65147-21-9
• Molecular Weight: 635.71
• UNSPSC Code: 12352204
• PubChem Substance ID: 24899025
• NACRES: NA.32

Properties

• Quality Segment: 200
• assay: ≥95%
• form: powder
• composition: Peptide content, ≥65%
• solubility: methanol: 50 mg/mL, clear, colorless to faintly yellow
• storage temp.: −20°C
• SMILES string: NC(NCCC[C@@H](C(NC1=CC=C(C(C)=CC(O2)=O)C2=C1)=O)NC([C@H](CC3=CC=CC=C3)NC(C4NCCC4)=O)=O)=N.CC([O-])=O
• InChI: 1S/C30H37N7O5.ClH/c1-18-15-26(38)42-25-17-20(11-12-21(18)25)35-28(40)23(10-6-14-34-30(31)32)36-29(41)24(16-19-7-3-2-4-8-19)37-27(39)22-9-5-13-33-22;/h2-4,7-8,11-12,15,17,22-24,33H,5-6,9-10,13-14,16H2,1H3,(H,35,40)(H,36,41)(H,37,39)(H4,31,32,34);1H
• InChI key: QYFPUTDFLKFOBA-UHFFFAOYSA-N

Description

General description

A fluorogenic substrate for pancreatic and urinary kallikreins.

Application

Pro-Phe-Arg-7-amido-4-methylcoumarin has been used as fluorogenic substrate:

• in kinetic experiments to measure the enzymatic activity of factor XII
• to measure the activity of generated plasma kallikrein using Spectramax plate reader
• to determine the cathepsin A (CatA) inhibitor selectivity against various proteases using Tecan Safire 2 plate reader

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)