P6675

Prolidase from porcine kidney

lyophilized powder, ≥100 units/mg protein

• Synonym(s): Aminoacyl-L-proline hydrolase, Imido Dipeptidase, Prolidase, Proline dipeptidase
• CAS Number: 9025-32-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-791-5
• MDL number: MFCD00132079
• EC Number: 3.4.13.9 (BRENDA, IUBMB)
• Specific activity: ≥100 units/mg protein

Properties

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥100 units/mg protein
• composition: Protein, 20-74% Lowry
• storage temp.: −20°C

Description

General description

Prolidase is a cytosolic exopeptidase. It is a homodimeric enzyme which requires divalent cations like manganese as a cofactor in its active site for its function.

Application

Prolidase from porcine kidney has been used:

• in the enzymatic hydrolysis of porcine milk for the recovery of L-glutamine from proteins and peptides
• in the proteolysis of skim milk for the determination of ε-(γ-glutamyl)lysine and free aminoacids
• to determine its effect on the activity of enterococcin A 2000

Prolidase has an important role in recycling of proline and collagen production. It is used to study mutations in the PEPD gene that cause prolidase deficiency. It is used to hydrolyze proteins with C-terminal proline or hydroxyproline residues. Prolidase, product P6675 from porcine kidney, has been used to hydrolyze peptide bonds from the amino terminus when studying enzymatic methylation of membrane proteins.

Biochem/physiol Actions

Rare mutation in prolidase gene causes deficiency leading to massive imidodipeptiduria, elevated proline-containing dipeptides in plasma, recurrent infections, mental retardation and skin lesions.

Prolidase is an enzyme that catalyzes the hydrolysis of the imide bond between an α-carboxyl group and proline or hydroxyproline. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues.

Physical form

Supplied as a lyophilized powder containing Tris buffer salt and MnCl₂.

Other Notes

One unit will hydrolyze 1.0 μmole of Gly-Pro per min at pH 8.0 at 40 °C.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1