The viscosity average molecular weight (Mv) is determined for each lot of this product. However, there is a distribution of molecular weights around that Mv. As a result, the molar concentration is not applicable. The typical degree of polymerization (DP) for this material is approximately 100 and the typical degree of substitution (moles of FITC per mole of lysine) is approximately 0.007 or 7 per 1000; essentially 7 of 10 Poly-L-Lysine molecules contain one FITC label. The concentration of the solution may be most accurately determined by precisely weighing the sample and precisely determining the volume, so that the sample may be reported as mg/mL. The degree of substitution and degree of polymerization are lot specific and reported in the product Certificate of Analysis. Please see the link below to review a sample of lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/p3543#product-documentation
P3543
Poly-L-lysine–FITC Labeled
mol wt 15,000-30,000
Synonym(s):
Fluorescein Labeled Poly-L-Lysine
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| Size/SKU | Availability | Price |
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About This Item
form
powder
Quality Segment
mol wt
precursor poly-L-lysine • HBr 15,000-30,000, 15,000-30,000
extent of labeling
0.003-0.01 mol FITC per mol lysine monomer
color
yellow to orange
storage temp.
−20°C
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| form powder | form powder | form powder or solid | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| color yellow to orange | color yellow to orange | color white to faint yellow | color white |
| extent of labeling 0.003-0.01 mol FITC per mol lysine monomer | extent of labeling 0.003-0.01 mol FITC per mol lysine monomer | extent of labeling - | extent of labeling - |
| mol wt precursor poly-L-lysine • HBr 15,000-30,000 | mol wt precursor poly-L-lysine • HBr 30,000-70,000 | mol wt >50,000 | mol wt 500-4,000 |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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ow do you determine the peptide concentration of a small scoop in solution? I am reading the absorbance at 495nm to read the fluorophore but I don't know how to convert that Absorbance value to a meaningful mg/mL or molarity concentration without an e.c.
1 answer-
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How much FITC quantity or weight percentage is present per mg of the mixture? If 10 mg of the product is weighed out, what portion of the weight corresponds to the PLL?
1 answer-
The FITC quantity present per mg of the mixture is 0.016 mg, which corresponds to approximately 1.6% of the weight. If 10 mg of the product is weighed out, the portion of the weight corresponding to the PLL is approximately 9.84 mg.
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