P350-05
Porcine Coronary Artery Smooth Muscle Cells: PCASMC (Cryovial)
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About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
Porcine coronary artery
Growth mode:
Adherent
Morphology:
smooth muscle
Relevant disease(s):
diabetes; stroke; cardiovascular diseases
form
solid
biological source
Porcine coronary artery
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 38
morphology
smooth muscle
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
diabetes; stroke; cardiovascular diseases
shipped in
dry ice
storage temp.
−196°C
Quality Level
Application
vascular research, supply of blood to heart muscle
Biochem/physiol Actions
Artery
General description
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PCASMC were used to investigate the role of c-Jun in smooth muscle proliferation, and to show that c-Jun inhibition abrogates smooth muscle cell growth and intimal thickening after arterial injury (Khachigian, 2002). PCASMC were also used to develop poly (l-lactide-co-caprolactone) nanofibrous scaffold material with potential for tissue engineering (Dong, 2008).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
PCASMC were used to investigate the role of c-Jun in smooth muscle proliferation, and to show that c-Jun inhibition abrogates smooth muscle cell growth and intimal thickening after arterial injury (Khachigian, 2002). PCASMC were also used to develop poly (l-lactide-co-caprolactone) nanofibrous scaffold material with potential for tissue engineering (Dong, 2008).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
Other Notes
Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
Preparation Note
- 2nd passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 16 doublings
Please refer to the PCASMC Culture Protocol.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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In Young Choi et al.
Biomaterials, 269, 120222-120222 (2020-08-02)
Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation
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