P3391

Protein A-Sepharose^™ from Staphylococcus aureus

lyophilized powder

• Synonym(s): Protein A–Agarose
• UNSPSC Code: 41106500
• NACRES: NA.56
• MDL number: MFCD00165563
• Form: lyophilized powder

Properties

• form: lyophilized powder
• Quality Segment: 200
• packaging: glass bottle of 1 g, glass bottle of 1.5 g, glass bottle of 250 mg, glass bottle of 5 g
• storage condition: do not freeze
• extent of labeling: 3 mg per mL
• technique(s): affinity chromatography: suitable
• color: white to gray
• matrix: Sepharose CL-4B
• matrix activation: cyanogen bromide
• matrix attachment: amino
• matrix spacer: 1 atom
• capacity: ≥16 mg/mL binding capacity (human IgG)
• solubility: water: 1 g/L at 20 °C
• suitability: conforms to structure for swelling capacity (1g swells to 4 to 5 mL)
• storage temp.: 2-8°C

Description

General description

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.

Application

Protein A-Sepharose was used:

• for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
• in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
• as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
• in immunoprecipitation of [³²P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.

Protein A-Sepharose^™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose^™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Physical form

Supplied as lyophilized powder stabilized with lactose and dextran.

Preparation Note

Swelling: 1 g swells to approx. 4 ml.

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)