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Peroxidase, Maleimide Activated from horseradish

Name: Peroxidase, Maleimide Activated from horseradish
Brand: SIGMA

lyophilized powder, >200 units/mg protein

Synonym(s):

Maleimide activated HRP

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

MDL number:

MFCD00284131

Specific activity:

>200 units/mg protein

Biological source:

horseradish

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Properties

biological source

horseradish

Quality Segment

200

form

lyophilized powder

specific activity

>200 units/mg protein

composition

Protein, ~50% E1%/403

extent of labeling

1-3 mol maleimide per mol protein

storage temp.

−20°C

Description

General description

Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as nicotinamide adenine dinucleotide (NAD) peroxidase, NADP peroxidase, fatty acid peroxidase and a group of non-specific enzymes from various sources. It is present in animals, higher plants and other organisms.

Application

For direct conjugation to compounds containing free sulfhydryl groups for use as an enzyme-linked detection reagent.
Instructions for use included. In a study on horseradish peroxidase, the Maleimide compounds succinimidyl 4-maleimidobutyrate and succinimidyl 6-maleimidohexanoate have been shown to be the most effective in the conjugation of Fab′ to horseradish peroxidase. [1] This enzyme has also been used in a study to investigate the effectiveness in staining a mitochondrial protein, lipoamide dehydrogenase. [2]

To increase signal intensity of immunoconjugates, immunoglobulins were synthetically modified to contain multiple primary amines and covalently coupled to maleimide activated peroxidase. This can result in more efficient conjugation and signal amplification. [3]

Biochem/physiol Actions

Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc.

Physical form

Lyophilized powder containing trehalose and sodium citrate

Other Notes

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 seconds at pH 6.0 at 20°C.

Type VI peroxidase activated with maleimidocaproic acid N-hydroxysuccinimide ester.

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This Item	11428861001	P2088	10108090001
Sigma-Aldrich P1709 Peroxidase, Maleimide Activated from horseradish	Roche 11428861001 Peroxidase (POD), activated	Sigma-Aldrich P2088 Peroxidase from horseradish	Roche 10108090001 Peroxidase (POD)
specific activity >200 units/mg protein	specific activity 800 units/mg protein (at 25 °C with ABTS and H₂O₂ as substrate (pH 5.0).)	specific activity 200-300 units/mg solid (using pyrogallol)	specific activity ≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg
biological source horseradish	biological source horseradish	biological source horseradish	biological source horseradish
form lyophilized powder	form lyophilized	form essentially salt-free, lyophilized powder	form lyophilized (salt-free)
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level -	Quality Level 300	Quality Level -
extent of labeling 1-3 mol maleimide per mol protein	extent of labeling -	extent of labeling -	extent of labeling -

pictograms

GHS08

signalword

Danger

hcodes

H317 - H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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