N3910

Neutralization Solution B

• Synonym(s): DNA neutralization solution, neutralization buffer DNA extraction
• NACRES: NA.25
• UNSPSC Code: 12161703

Properties

• grade: Molecular Biology
• Quality Segment: 200
• form: liquid
• feature: Direct PCR
• technique(s): DNA amplification: suitable, DNA extraction: suitable, PCR: suitable
• input: crude DNA
• storage temp.: −20°C

Description

General description

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp^™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Application

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp^™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp^™ PCR Reaction Mix.[1]

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H315,H317,H319,H411
• pcodes: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids