Specific information on the measurement uncertainty of this product is not available; however, during the development of these kits, an error of less than 10% is targeted, with all measurements performed in duplicate. Therefore, a fairly low measurement of uncertainty can generally be expected when running tests. The standard curve is also run with every group of samples to normalize results and obtain more consistent data.
MAK443
L-Lactate Assay Kit
Sufficient for 100 fluorometric tests
Synonym(s):
ʟ-Lactic Acid Assay Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Pricing and availability is not currently available.
Quality Segment
input
cell culture medium
plasma
serum
urine
detection method
fluorometric
relevant disease(s)
diabetes; pulmonary disorders; endocrinological disorders; cardiovascular diseases; cancer
storage temp.
−20°C
General description
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.
Application
The L-Lactate Assay Kit may be used for:
- Lactate Metabolism Research
- Tissue Metabolism Research
- Drug Discovery
The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.
Biochem/physiol Actions
The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.
Features and Benefits
- Simplified Process: Experience a streamlined process with the addition of only a single working reagent, reducing complexity and saving valuable time and effort.
- Broader Detection Range: Detect L-Lactate levels accurately across a wide range, from 1 to 50 μM, using just 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
- High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, ensuring seamless operation and maintaining efficiency and accuracy in large-scale analyses.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
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This Item | |||
|---|---|---|---|
| Quality Level 100 | Quality Level - | Quality Level - | Quality Level 100 |
| detection method fluorometric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| input cell culture medium | input cell medium | input cell | input - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) diabetes; pulmonary disorders; endocrinological disorders; cardiovascular diseases; cancer | relevant disease(s) neurological disorders; gastrointestinal diseases | relevant disease(s) endocrinological disorders, diabetes; cancer | relevant disease(s) diabetes; cancer; endocrinological disorders |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
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Could you provide information on the measurement uncertainty?
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Helpful?
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The kit contains a probe, but no instructions in the protocol of when/how much to add. Please advise on the probe-adding step?
1 answer-
The protocol indicates that 5 uL of the probe should be added to the working reagent. This is described in Table 2, located on page 3 of the datasheet. Please see the link below to review:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/429/213/mak443pis-ms.pdfHelpful?
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