This product is discontinued. However, the original stock concentration in the inhibitor component is 100 mM Clavulanic Acid.
MAK222
Beta-Lactamase Inhibitor Screening Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
β-Lactamase Inhibitor Screening Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Pricing and availability is not currently available.
detection method
colorimetric
storage temp.
−20°C
General description
β-Lactamase (β-lactamase, βL, EC 3.5.2.6) is an enzyme first identified in Escherichia coli and has been described as penicillinase. A number of βLs have since been identified from various bacteria. β-Lactamases specifically hydrolyze β-lactam rings present in antibiotics such as penicillin, cephalosporins, monobactam, and carbapenem, and confer resistance against these antibiotics.
Application
Beta-Lactamase Inhibitor Screening Kit has been used in enzymatic assays.
Suitable for the screening of inhibitors of β-Lactamase
Biochem/physiol Actions
The β-Lactamase Inhibitor Screening Kit is a rapid, simple and sensitive assay that is suitable for high throughput screening of β-Lactamase inhibitors. βL activity is measured by hydrolyzing a chromogenic cephalosporin called nitrocefin, producing a colorimetric product (A490), proportional to the enzymatic activity present.
Features and Benefits
Compatible with high-throughput handling systems.
Kit Components Only
Product No.
Description
- β-Lactamase Assay Buffer
- Nitrocefin, in DMSO
- β-Lactamase
- Inhibitor Control, Clavulanic acid
Signal Word
Danger
Storage Class
10 - Combustible liquids
Hazard Codes
Precautionary Statements
Hazard Classifications
ED ENV 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1
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What is the concentration of clavulanic acid?
1 answer-
Helpful?
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Hello, I want to use your β-lactamase inhibitor screening kit, however I could not find which β-lactamase(s) the kit includes, and where I can a step by step protocol
1 answer-
Here is a copy of the protocol for this product: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/767/737/mak222pis-ms.pdf
Included are nitrocefin, β-lactamase, a β-lactamase assay buffer, and the inhibitor control clavulanic acid. These are not sold as distinct products on the website.
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