MAK150
Fluorometric Intracellular pH Assay Kit
sufficient for 1000 fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 1000 fluorometric tests
detection method
fluorometric
relevant disease(s)
neurological disorders; genitourinary disorders; cancer
storage temp.
−20°C
General description
Intracellular pH changes are implicated in diverse physiological and pathological processes, including cell proliferation, apoptosis, fertilization, malignancy, multidrug resistance, ion transport, lysosomal storage disorders, and Alzheimer′s disease.
This kit can be utilized to measure decreases in intracellular pH in cells treated under various conditions using a simple standard protocol. Alternatively, an Acid-Load procedure can be used to measure the increase of intracellular pH associated with changes in cellular metabolism due to GPCR activation, growth factor activity, or other cellular stimulation. With the Acid-Load procedure ammonium chloride solution is added after the fluorescent pH dye is loaded into cells in a minimum volume. This Acid-Loading step is followed by the addition of agonist in a relatively large volume (~4x) of buffer. The sudden volume change initiates an efflux of ammonia (NH3) from the cells causing a rapid decrease in intracellular pH, and thus a decrease in fluorescence signal. The effect of agonist on the subsequent recovery of intracellular pH is measured by the relative fluorescence signal increase.
This kit can be utilized to measure decreases in intracellular pH in cells treated under various conditions using a simple standard protocol. Alternatively, an Acid-Load procedure can be used to measure the increase of intracellular pH associated with changes in cellular metabolism due to GPCR activation, growth factor activity, or other cellular stimulation. With the Acid-Load procedure ammonium chloride solution is added after the fluorescent pH dye is loaded into cells in a minimum volume. This Acid-Loading step is followed by the addition of agonist in a relatively large volume (~4x) of buffer. The sudden volume change initiates an efflux of ammonia (NH3) from the cells causing a rapid decrease in intracellular pH, and thus a decrease in fluorescence signal. The effect of agonist on the subsequent recovery of intracellular pH is measured by the relative fluorescence signal increase.
Application
Fluorometric Intracellular pH Assay Kit has been used to measure the intracellular pH.
Features and Benefits
Superior alternative to BCECF
Suitability
This kit can be utilized to measure changes in intracellular pH in cells treated under various conditions.
Principle
The Fluorometric Intracellular pH Assay Kit utilizes a proprietary cell-permeable fluorescent indicator, BCFL-AM, for measuring relative intracellular pH changes. BCFL-AM is a superior alternative to the popular ratiometric pH probe, 2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Unlike BCECF, which is a complex and variable mixture of isomers, BCFL-AM is a single isomer species. BCFL-AM has the same spectral responses as BCECF-AM (λex = 490 nm/λem = 535 nm for single measurement or λex = 505 nm/λem = 535 nm and λex = 430 nm/λem = 535 nm for ratio measurement) but enhanced reproducibility. BCFL-AM exhibits pH-dependent dual excitability, pKa ~7.0, and an excitation isosbestic point of 454 nm.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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