MAK088

DPP4 Activity Assay Kit

sufficient for 100 fluorometric tests

• Synonym(s): CD26 Activity Assay Kit, Deaminase Complexing Protein 2 Activity Assay Kit, Dipeptidyl Peptidase-4 Activity Assay Kit
• EC Number: 200-664-3
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 fluorometric tests
• detection method: fluorometric
• storage temp.: −20°C
• Gene Information: human ... DPP4(1803) ; mouse ... DPP4(13482) ; rat ... DPP4(25253)

Description

General description

Dipeptidyl peptidase-4 (DPP4), also known as CD26 and adenosine deaminase complexing protein 2, is a serine exopeptidase that cleaves X-Proline and X-Alanine residues from the N-termini of polypeptides. DPP4 is a transmembrane glycoprotein whose activity regulates the bioactivity of multiple peptides such as growth factors, chemokines, and neuropeptides. DPP4 plays a major role in glucose metabolism via the regulation of glucagon-like peptide-1 and inhibitors of DPP4 are commonly used for the treatment of type 2 diabetes. DPP4 also plays an important role in immune regulation and may play a role in tumor suppression. Deletion of the DPP4 gene affects carbohydrate metabolism which in turn leads to mental retardation and hypotonia. Abnormal expression of the protein is observed during liver regeneration, cirrhosis and liver tumorigenesis.

Application

DPP4 Activity Assay Kit has been used to determine the activity of dipeptidyl peptidase-4 (DPP4) in homogenates.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of dipeptidyl peptidase-4 (DPP4) in a variety of samples including tissue, cells and serum

Principle

The DPP4 Activity Assay kit provides a simple and direct procedure for measuring DPP4 activity in a variety of samples. In this assay, DPP4 cleaves a non-fluorescent substrate, H-Gly-Pro-AMC, to release a fluorescent product, 7-Amino-4-Methyl Coumarin (AMC) (λ_ex = 360/λ_em = 460 nm). One unit of DPP4 is the amount of enzyme that will hydrolyze the DPP4 substrate to yield 1.0 μmole of AMC per minute at 37 °C.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup