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M9288

Sigma-Aldrich

Minimum Essential Medium Eagle

With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Minimum Essential Medium Eagle, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
HEPES: no
sodium pyruvate: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Minimum Essential Medium (MEM), one of the most used synthetic cell culture media, is developed by Harry Eagle. It is used to cultivate various types of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used as a transport media to maintain pig liver cells.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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L González-Valero et al.
Animal : an international journal of animal bioscience, 8(11), 1873-1880 (2014-07-16)
There are important differences in terms of metabolic activity, energy utilization and capacity of protein and fat deposition when Iberian and modern pigs are compared. Primary culture of hepatocytes was used to evaluate hepatic function and sensitivity to hormones between
Simona Giunta et al.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events
Hisashi Miura et al.
Nucleic acids research, 37(3), 778-792 (2008-12-17)
Mammalian tissue- and/or time-specific transcription is primarily regulated in a combinatorial fashion through interactions between a specific set of transcriptional regulatory factors (TRFs) and their cognate cis-regulatory elements located in the regulatory regions. In exploring the DNA regions and TRFs
Barbara Zonta et al.
Neuron, 69(5), 945-956 (2011-03-09)
The axon initial segment (AIS) is critical for the initiation and propagation of action potentials. Assembly of the AIS requires interactions between scaffolding molecules and voltage-gated sodium channels, but the molecular mechanisms that stabilize the AIS are poorly understood. The
Natsumi Shimizu et al.
International journal of hematology, 87(3), 239-245 (2008-03-05)
Although coculture of hematopoietic stem cells (HSCs) with stromal cells is a useful system to study hematopoiesis in the niche, little is known regarding the precise cellular and molecular mechanisms of maintaining HSCs through cell-cell interactions. The murine preadipose stromal

Articles

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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