Unfortunately, no protocol is available for this product. References can be found in "Methods of Soil Enzymology, Volume 9, chapter 14: Microplate Fluorimetric Assay of Soil Enzymes."
M7133
4-Methylumbelliferyl sulfate potassium salt
sulfatase substrate
Synonym(s):
Potassium 4-methylumbelliferyl sulfate
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About This Item
Empirical Formula (Hill Notation):
C10H7KO6S
CAS Number:
Molecular Weight:
294.32
UNSPSC Code:
12352204
NACRES:
NA.32
PubChem Substance ID:
EC Number:
239-272-2
Beilstein/REAXYS Number:
3803203
MDL number:
Pricing and availability is not currently available.
Quality Level
assay
≥99% (HPLC)
form
powder
solubility
water: 5 mg/mL, clear, colorless
fluorescence
λex 334 nm; λem 370 nm (pH 10.4), λex 360 nm; λem 449 nm (Reaction products)
storage temp.
−20°C
SMILES string
[K+].CC1=CC(=O)Oc2cc(OS([O-])(=O)=O)ccc12
InChI
1S/C10H8O6S.K/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;/h2-5H,1H3,(H,12,13,14);/q;+1/p-1
InChI key
CSOCSPXOODWGLJ-UHFFFAOYSA-M
General description
Application
4-Methylumbelliferyl sulfate potassium salt has been used as a substrate in coupled fluorescence sulfotransferase assay to regenerate 3′-phosphoadenosine 5′-phosphosulfate (PAPS) from 3′-phosphoadenosine 5′-phosphate for cerebroside sulfotransferase (CST) enzyme activity measurements.[3] It has also been used as a substrate in arylsulfatase assay/4-methylumbelliferyl sulfate (4-MUS) assay to test the enzymatic activity of the protein-bead complex.[1]
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This Item | |||
|---|---|---|---|
| assay ≥99% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (TLC) | assay ≥98% (HPLC) |
| solubility water: 5 mg/mL, clear, colorless | solubility H2O: 50 mg/mL | solubility water: 50 mg/mL, clear, colorless | solubility water: 20 mg/mL |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 100 |
| form powder | form powder | form powder | form crystalline solid |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| fluorescence λex 334 nm; λem 370 nm (pH 10.4), λex 360 nm; λem 449 nm (Reaction products) | fluorescence - | fluorescence λex 319 nm; λem 384 nm (pH 9.0), λex 360 nm; λem 449 nm (Reaction product) | fluorescence λex 360 nm, λem 440 nm |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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A M Hassen et al.
Drug metabolism and disposition: the biological fate of chemicals, 24(7), 792-798 (1996-07-01)
The uptake of estrone sulfate (E1S; 1 to 400 microM), harmol sulfate (HS; 5 to 900 microM), and 4-methylumbelliferyl sulfate (4MUS; 5 to 1000 microM) was investigated in isolated rat hepatocytes in the presence or absence of inhibitors. Uptake of
O P van Diggelen et al.
Journal of inherited metabolic disease, 12(3), 273-280 (1989-01-01)
Arylsulphatase C (ASC) activity in leukocytes and fibroblasts measured with 4-methylumbelliferylsulphate, is caused by at least two genetically different sulphatases. One of these is steroid sulphatase (STS). Depending on the substrate concentration, about 10-50% of the ASC activity in leukocytes
C L Zimmerman et al.
Journal of chromatography, 563(1), 83-94 (1991-01-18)
A direct high-performance liquid chromatographic (HPLC) assay was developed for the separation and determination of 4-methylumbelliferone (4MU) and its glucuronide (MUG) and sulfate (MUS) conjugates in the cell-free perfusate ("plasma") from in situ perfused rat intestine-liver preparation. In addition, a
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M7133-1G | 04061834061649 |
| M7133-500MG | 04061834061656 |
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Bonjour, Je souhaite mesurer par fluorescence l'activité arylsulfatase dans des sols au moyen de votre substrat M7133. Avez vous un protocole etali svp? Merci d'avance.
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