M6770

MCDB 201 Medium

With trace elements, L-glutamine and 30 mM HEPES, powder, suitable for cell culture

• Synonym(s): MCDB medium
• NACRES: NA.75
• UNSPSC Code: 41161501

Properties

• form: powder
• technique(s): cell culture | mammalian: suitable
• components: HEPES: 7.149 g/L (30mM); glucose: 1.441 g/L (Dextro); phenol red: 0.001242 g/L; sodium pyruvate: 0.055 g/L; L-glutamine: 0.14615 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

MCDB 201 medium is a modified Ham′s nutrient mixture F-12 that is synthesized for the clonal growth of chicken embryo fibroblasts.

Application

MCDB 201 Medium has been used as a component of:

• the hepatic differentiation medium to culture pluripotent stem cells,
• epididymal growth medium for culturing of preadipocytes,
• Dulbecco′s Modified Eagle Medium (DMEM)/F12 for culturing of human umbilical cord mesenchymal stem cells

Preparation Note

Formulated to contain 17.7 grams of powder per liter of medium.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable