M6518

Macrophage Colony-Stimulating Factor human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): MCSF
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00166591
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: dimer 37 kDa
• Impurities: ≤1.000 EU/μg

Properties

• Product Name: Macrophage Colony-Stimulating Factor human, M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• quality: endotoxin tested
• mol wt: dimer 37 kDa
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.000 EU/μg
• color: white
• suitability: suitable for (≤5.000 ng/ml (ED50))
• UniProt accession no.: P09603
• storage temp.: −20°C

Description

General description

Macrophage Colony-Stimulating Factor (M-CSF) is a hematopoietic growth factor, which modulates the proliferation, differentiation and functional activation of monocytes. It is produced by fibroblasts, endothelial cells and bone marrow stromal cells. MCSF stimulates the functions of mature monocytes and macrophages.

Application

Macrophage Colony-Stimulating Factor human has been used for the differentiation of bone marrow-derived macrophages (BMDMs) and human monocyte-derived macrophages (MDMs). It has also been used for cell culture.

Biochem/physiol Actions

Macrophage colony-stimulating factor (M-CSF) augments many of the physiological functions of macrophages, and support osteoclast differentiation.

Physical form

Lyophilized from a sterile filtered buffered solution.

Analysis Note

The proliferative activity is tested in culture using the cytokine-dependent murine monocytic cell line M-NFS-60 cells.

Other Notes

159 amino acids including N-terminal methionine (each monomer).

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)