M2520

Medium 199

HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): M199 Medium, TCM 199
• NACRES: NA.75
• UNSPSC Code: 12352207

Properties

• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: L-glutamine: yes; phenol red: yes; Earle’s salts (5% CO₂): yes; NaHCO₃: no; sodium pyruvate: no; HEPES: 25 mM
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used to:

• maintain the cumulus-oocyte complex (COC) before maturation and for culturing of these oocytes for in vitro maturation
• to rinse the aspiration line during the collection of the ovum from German Holstein cows
• to culture cardiac endothelial cells from rats

Preparation Note

Formulated to contain 14.7 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable